I just found strange problem with animate package and lualatex. When I compile the same latex file with pdflatex, the animation in the PDF runs ok. When I compile with lualatex the animation does not work (but the image is there) but clicking on the play button does nothing. I am simply using
\animategraphics[controls,loop,width=6.5in,buttonsize=2em]{2}{matlab/frame}{1}{100}
I am trying to find if this is known issue. Will keep googling. The files are all png format