with no rule changes the time of no disturbance required, after which all everything can be lost, becomes very important.
For 1 food surrounded by 80 fungi that's 829 frames (but in the 30k frames there'll be absolutely no food gain)
For a 16x16 checkerboard it's 2209 (1 food/17.3 frames)
For a 16x16 checkerboard surrounded by 3 layers of fungi - 1353 frames (1 food/10.5 frames, quite an improvement from above)