would "swirl to mix" be synonymous with "mix in a circular motion"?

@CowperKettle Yes

@SanjuktaGhosh Thank you! Dhanyavaad! (0:

> The development of color takes 20–25 min in a light-protected environment at (21±1) °C. (I'm translating an ELISA procedure)

Is this the usual way of placing a temperature range into round brackets?

In Russian I come across this now and then. Not sure about English.

@WYSIWYG they can be called "popup windows" or "dialog boxes" or just plain "windows"

@CowperKettle I'm not sure the parentheses (round brackets) are necessary here. Also, is the text in question referring to an HRP-mediated reaction? In that case, protection from light is not necessary. It's just a common lab urban legend.

You can do the development in the dark and it won't hurt anything, but it's not necessary. Neither the enzyme nor the substrate chemicals will lose any detectable activity in that short of a period of time.

@MattDMo I did not know that! But I only glanced across the procedure on some PDFs a couple of times.

Probably my authors cling to this urban legend to be on the safe side.

@CowperKettle I literally can't count the number of ELISAs I've done in my life - probably somewhere in the mid-thousands if I had to guess. It's just one of those things you pick up on after a while - you accidentally leave a plate out on the bench during development, and it works exactly the same as the plate you ran yesterday!

@CowperKettle most likely. If they're using the standard TMB two-part substrate, I believe one of the components is somewhat light-sensitive, which is one reason why they ship in opaque bottles. However, it would have to be exposed to bright light for a very long time (days to weeks to months) before you'd see any effect in your assays.

I recall the mention of TMB

I don't know whether it's two-part

@CowperKettle

> Dhanyavaad!

:)

(0:

Namaste! (0:

:D :D

Yeah. Namaskar.

@CowperKettle

It is a bengali word for Namaste (that's Hindi).

Zdorovo!

It's the Russian word for "Hi!"

@CowperKettle In Bengal we don't use Namaskar that often. We just greet with (Kamon acho) How do you do?

You are from Russia?

Yes

I see.

And in Russia too.

:)

(0:

Nice to meet you. Got to go. Have to study.

Oh. Study is great. Have a great study time, Sanjukta!

My authors use sodium acetate + TMB. This could be a "two-part" substrate

They call the NaOAc-based solution "substrate buffer"