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Sanjukta Ghosh
Sanjukta Ghosh
9:40 AM
@AhmetÖz Welcome to biostack exchange.

Please go through some questions under the tag book-recommendation so that you know how to narrow down a broad opinion based question as the one you mentioned.

Here's a good example :http://biology.stackexchange.com/questions/39003/introductory-book-on-cell-biology

and this this the link to the tag info: http://biology.stackexchange.com/tags/book-recommendation/info
 
 
2 hours later…
CowperKettle
CowperKettle
11:11 AM
Can one cultivate a cell line at 150 RPM in a shaker incubator?
I've just come across this in my Certificate of Analysis document
Section "cultivation conditions"
Would not cells be too disturbed when rotated at such a speed?
 
 
1 hour later…
Ahmet Öz
Ahmet Öz
12:24 PM
@SanjuktaGhosh
Thank you so much :) I asked ı hope someone answer :D
 
Sanjukta Ghosh
Sanjukta Ghosh
@AhmetÖz

Good luck.
 
 
10 hours later…
MattDMo
MattDMo
10:51 PM
cv-pls One more close vote needed for this one - homework, too broad, would much rather argue about definition of homework than research his own question, etc.
@CowperKettle sure. Bacteria are grown under those conditions all the time, and so are protein-producing mammalian cell lines like CHO, 293-S, etc. In these cases the shear stress can actually aid in protein production. It also prevents the cells from falling to the bottom of the flask, clumping, and being starved of nutrients.
 

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