The Biosphere

6:28 AM

Is "nebulizer gas" the same as "sheath gas" in LC-MS?

5 hours later…

11:25 AM

I wonder what Activation Q is in Mass Spectrometry

12:23 PM

@CowperKettle give me a few hours to get to work and I'll let you know.

@MattDMo What'S your local time? Morning I guess?

@MattDMo Thank you, Matt! I seems to have found out.

> Activation q: the energy that is responsible for accelerating all of the ions to be fragmented. (although I don't quite fully understand this, at least I have some hazy idea)

12:44 PM

*seem

1 hour later…

2:00 PM

Is "Protein A affinity sorbent" = a sorbent that binds Protein A, or = a sorbent that includes Protein A as its integral part?

> Residual Protein A may appear in the drug substance due to its leaching when the ligand is being cleaved from the affinity sorbent.

ncbi.nlm.nih.gov/pubmed/17643441

Quite interesting.

Never thought that it's so complex.

I guessed the right word, "leaching"

@Chris US East coast, currently with Daylight Savings Time it's UTC -04:00. It is currently 10:08 AM, according to my laptop.

Ok, six hours behind me. My workday is almost over. :-)

19:11 here (0:

Lucky. I've got staining and cell splitting and freezing (maybe) and other fun stuff planned for today. Guess I better check in with my boss to see what he thinks I should be doing :)

cell splitting?

by bead milling?

2:11 PM

@CowperKettle did you figure out the nebulizer gas thing?

@MattDMo I used the word "nebulizer gas" and went further in the translation

I downloaded 500 mb of books for later perusal

@CowperKettle No, cell culture. I'm growing cell lines in vitro, and once they become confluent I split the culture so they can continue to grow.

@MattDMo I have gone through that. Although my boss is off to a conference for the rest of the week. So some kind of relaxation now :-)

@MattDMo Ah!

or split some cells off, in case you want to do experiments with them

2:12 PM

that too.

but since I am on a conference next week and have the week of after that, I have reduced my cell culture from 12 lines to only 4.

doesn't take me a whole morning plus to get through there.

I have two lines - one is an immortalized B cell line from a patient with a genetic disease, the other is from his brother, who does not have the gene of interest. I'm going to be running flow cytometry with several different antibodies to see if I can differentiate between them.

Oh, and some HeLas, but they don't really count :)

no, these simply grow.

and grow and grow and grow...

I keep my fingers crossed that I will not get any tumor tissue for the rest of the week from which I have to isolate cells.

Hela. Growing since 1953.

Together with an image this could be a nice T-Shirt slogan.

2:17 PM

@MattDMo Cool

how about this one?

Nice one. I only have images from sple bright field microscopy.

@MattDMo What did you stain there? Dapi (or something similar) for the nucleus is obvious, but what about the rest?

It's not mine, I found it on Wikipedia.

From the description, they used phalloidin (red), microtubules (cyan), and some sort of DNA dye (blue).

It's originally from NIH, but I can't find any more info on which antibodies/dyes they used.

OK, I gotta get to work. Talk to you all later!

Have fun.

This one is also nice:

2 hours later…

4:06 PM

A fellow woman translator is unsure whether she can use "C-terminal lysine truncation" and "C-terminal lysine processing" as fully synonymous phrases. This is in relation to monoclonal antibodies used in pharmacology.

ncbi.nlm.nih.gov/pubmed/20830675

It looks like these lysine residues are truncated from the antibody, but still "processing" is a widely used word

terdon

@CowperKettle Why is the person's gender relevant?

@terdon Is it? I just mentioned it.

terdon

It just seems odd that you felt it necessary to specify that this other translator is a woman, that's all.