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Secret
Secret
7:43 AM
I see opportunities besides medical for this finding. It might be relevant to biofilm and microbiome engineering as there might be similar molecules used by aerobic bacteria to survive in anaerobic communities and other harsh conditions. Imagine injecting some phenazines into a community. Perhaps this will help the community to better thrive and thus provide more control on the biofilm structure for certain industrial applications
 
 
2 hours later…
Secret
Secret
9:34 AM
and this one talks about certain peptide sequences can abort translation of some ribosomes in E coil., and this behaviour is exploited by the bacteria to use as a Mg2+ sensor
 
CowperKettle
CowperKettle
10:23 AM
MALDI imaging
MALDI imaging mass spectrometry (MALDI-IMS) is the use of matrix-assisted laser desorption ionization as a mass spectrometry imaging technique in which the sample, often a thin tissue section, is moved in two dimensions while the mass spectrum is recorded. Advantages, like measuring the distribution of a large amount of analytes at one time without destroying the sample, make it a useful method in tissue-based study. == Sample preparation == Sample preparation is a critical step in imaging spectroscopy. Scientists take thin tissue slices mounted on conductive microscope slides and apply a suitable...
I don't understand how this works.
MALDI is supposed to ionize the source, so how come we can perform imaging of it?
It is supposed to become a stream of vaporized particles
You can hardly image that
The Wikipedia article is cryptic
 
Secret
Secret
The vaporised particles entered the mass spectrometer, which the identity of the molecules can then be identified
Since the vaporised particles are collected at the location where they are vaporised, it provides location based data of each type of (ionisable) chemical species on the sample
 
CowperKettle
CowperKettle
So they use the computer to "restore" the picture. Ah. Thank you!
They fire the laser at a point, record its location, then see what was in that point, and so on, until the sample is destroyed
> Air-dry the samples.
I wonder what the alternative phrase might be.
> Leave the samples on air (??) to dry
Hmmm.
 
Secret
Secret
in air?
 
 
4 hours later…
CowperKettle
CowperKettle
2:27 PM
Thanks!
How does one formulate this
> Mix the solution on / using a vortex mixer?
Is it okay to say
> Vortex-mix the solution
 
 
2 hours later…
CowperKettle
CowperKettle
4:28 PM
Is the acronym PAAG widely used and understood to mean polyacrylamide gel?
 
canadianer
canadianer
4:42 PM
@CowperKettle I would likely say "mix the solution with a vortex mixer" or "vortex the solution".
I've never heard PAAG used before. PAGE is the standard abbreviation for polyacrylamide gel electrophoresis. If you do use it, make sure you define it at the first instance and probably include a table in the paper listing all abbreviations used.
 

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