I think it's time we just accepted that.

Hell, even the site's description states that we're for people studying biology at any level:

> Biology Stack Exchange is for people studying biology at any level. Questions are from students, academics and active researchers in biology and closely related fields, including:

However, the fact is that we get very, very few expert-level questions. I don't have stats handy, but I'd be very surprised if even 10% of the questions on the site are even at the undergrad level, let alone professional.

If we were to change the rules somehow to only allow questions from people actually in the field, I think the site would just shrivel up and die. There just aren't enough of us asking questions.

I, for example, have only asked 12 and you've only asked 10, while I've answered 158 and you 168. That gives you a general idea of how often "experts" ask here, I think.

The graph shows the number of questions asked per reputation. As you can see, most questions are asked by low rep users.

Huh. Chemistry is even worse:

Oh wow, Physics seems awful:

So, there seems to be a general trend in the science sites that most questions are asked by low rep users. This, of course, doesn't necessarily imply that they're not experts, but it might indicate it.

I think a rough grouping would help here, e.g. grouping by a few privilege levels: Number of questions asked by 1 rep, <101 rep, <1k, <3k, < 10k.

@MadScientist Yeah, it's hard to get any clear conclusions from this. I just wanted a rough approximation

@MadScientist there you go. That's the total number of questions asked by users with rep <= N, where N ranges from 1000 to 30000 at intervals of 1000.

The effect looks even more extreme in this graph

Yep

Oh, and my title isn't very clear. I plotted each rep range from 1000 to 30000. So, the first data point is users with 0< rep <= 1000, the second is 1000< rep <= 2000 and so on.

We only have 13.000 questions, the first data point looks like almost all of them are part of it

Ah, yes. I must have screwed up somewhere, let me check again.

Hang on, isn't the 13.000 only open questions? I think the data.se query I used is counting everything.

Are you querying the set with deleted posts, or the regular one?

@MadScientist data.stackexchange.com/biology/query/524157/…

Everything AFAIK

I just checked and it also counts closed, not sure about deleted.

There is a PostsWithDeleted table, but I'm not sure if that would work here as you also need to link the user, and that table does have some information removed

OK, yes, I had a bug, This one should be correct and is even worse:

The size of the points reflects the number of questions asked.

Heh, that bump at ~27K rep is Remi with his ~200 questions :)

Good evening!

> The quantity of the assessed messenger RNA (mRNA) is normalized to an amplified housekeeping gene in each of the compared samples, after which the ΔΔCt value is calculated. The procedure uses tRNA obtained from cells of the main bank of the producing strain as its reference standard.

Does this look logical?

I don't understand how tRNA could be used there.

Is the question worthy of asking on the main site?

I know too little of qPCR (I suppose the paragraph is about qPCR)

The difference between the immunology and immune-system tag seems trivial. Is it worthy of a tag? Mods pls check out?