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Q: How can we determine the purity of cells isolated from Rat brains if we cannot use FACS, Immunohistochemistry or SEM anaysis?

subhash narasimhanPlease help I know how to isolate the different cells from a brain tissue(Ficoll based separation) (astro, other glial cells, neurons and synaptosomes) but,how do i determinre the purity of the extract that i have isolated if i cannot use immunological techniques, FACS or SEM analysis.

 

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