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another 'Homo sapien'
another 'Homo sapien'
4:23 AM
@Chris :-) 👍
 
 
3 hours later…
CowperKettle
CowperKettle
7:32 AM
Is mini vortex mixer a good generic term to describe a vortex mixer that accepts only a single tube?
 
CowperKettle
CowperKettle
8:27 AM
If I name this thing "Anti-CHO-HCP: HRP Conjugate" in a table, would this be understood correctly?
 
WYSIWYG
WYSIWYG
8:44 AM
@CowperKettle I guess most vortex mixers accept only a single tube. The ones that I have used don't even have any feature that really "accepts" a tube. You just have to hold the tube in hand while vortexing. :P
 
CowperKettle
CowperKettle
@WYSIWYG Thank you! The Wikipedia article misguided me by telling that there are vortex mixers that accept plates
> If the concentration of the drug in a sample does not exceed 1.0 mg/ml, transfer 500 µl of the sample into a 1.5 ml microtube, add 200 µl of solution 7 for dilution of samples and mix (test solution).
I wonder if the English reader will understand this: we have created the test solution thus.
It is the custom in Russian to mark in round brackets what we have just prepared.
 
WYSIWYG
WYSIWYG
9:47 AM
@CowperKettle Oh there are mixers that hold plates too. There are custom mixers for all kinds of containers but I haven't seen vortex mixers for plates.
 
 
2 hours later…
CowperKettle
CowperKettle
11:37 AM
Adjust the pH of the solution to (8.0±0.1) with concentration hydrochloric acid (by potentiometry).
Would this be understood as "control your adjustment process using a potentiometer"?
Maybe there's a better formulation.
Just "(potentiometry)"?
A lot of Russian sentences in procedure descriptions have this parenthetical (potentiometry), and I don't know how to translate this.
 
 
6 hours later…
CowperKettle
CowperKettle
5:22 PM
@MattDMo - thank you for the comment. At least on main site it is more livelier in terms of answers.
> 10-fold concentrated buffer solution for dilution of samples
Would this be an okay description, I wonder.
> 10X concentrate of the buffer solution for dilution of samples
> 10X concentrate of the sample dilution buffer
I favor the last variant
Is this a universal, common notation in chemistry: 10X for a ten-fold concentrate?
>In a molecular biology research lab, you will constantly need to make and use buffers. In
order to save time and space, molecular biologists often make concentrated stocks of solutions to
last over long periods of time. Such concentrated stocks take up less space. In addition, these
stocks are easily diluted for use when necessary. In this and other labs, you will often deal with
solutions that are labeled “10X,” “5X,” “100X,” etc. It is important to understand what this “X”
factor means.


The “X” factor simply indicates that the solution is in a concentrated form that must
 
 
2 hours later…
MattDMo
MattDMo
7:05 PM
@CowperKettle yup, this one is best
@CowperKettle in all the labs I've been in, yes. Granted, I haven't worked in a full straight-out chem lab since my undergraduate days almost 20 years ago, but I've spent plenty of time in bio and biochem labs, and the X abbreviation is used universally, both for self-prepared solutions and those obtained from vendors. I would assume straight chem would be the same, but I just asked in The Periodic Table just to make sure.
 
Mad Scientist
Mad Scientist
10X is a very common notation in bio/biochem labs. I don't think chemists tend to use it much, but mostly because they don't often need this kind of stock solution. But it's really common e.g. for enzyme buffers, or for stuff like electrophoresis buffer where you want a highly-concentrated stock simply because it takes up much less space than the 1X solution
 
MattDMo
MattDMo
@CowperKettle regarding the last sentence: that's not necessarily true, so don't take that at face value. For example, in an ELISA assay, I may be using a base 1X sample buffer (PBS with 0.05% Tween-20 or PBS-T) and the 1000X detection antibody needs to be diluted in that, not water.
 

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