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I was trying to measure standard solution of CY5 (at $\pu{649 nm}$; concentrations: $10, 5,$ and $\pu{1 \mu M}$) and Alexa 488 (at $\pu{496 nm}$; concentrations: $20, 10, 5 ,2.5,$ and $\pu{1 \mu M}$) by absorbance (separately), but I couldn't get any difference between any solution just like the ...