The Biosphere

1:21 AM

@AtlLED Not in general, no. They need to have been in the specific chat room within the last two weeks, afaik. I'm not sure if this rules applies to all chat rooms, though.

3:03 AM

@Chris and @WYSIWYG - I edited the question: biology.stackexchange.com/questions/34620/…

This in answer to @aandrev's remark : "can we probably add a disclaimer that question is not for discussing vaccines, but for providing credible references that show harmful effects of vaccination?"

3:49 AM

I wonder if this site has any creationism questions. E.g. We are taught the world was created in 4004 BC. How do we know it wasn't created in 4000 BC? It's a nice round number.

5:51 AM

@FaheemMitha yeah people do ask questions on creationism. We promptly close them :P

6:08 AM

PRIVILIGES !!!!!!!!!!!!!!!!

@AliceD Haha.. Do you feel like a deposed emperor ??

6:25 AM

@WYSIWYG OOOOhhhhh YYEEEAAAHHHH

@AliceD Suggest me an interesting paper.. I am too bored

not some high-level paper on neuroanatomy and all :P

Q: SDS Laemmli Gel that did not solidify

7:05 AM

5

In my lab class this week my partner and I were making a SDS-PAGE Laemmli Gel. We made the resolving gel solution which was made of 30% Acrylamide, DI water, 1.5 M Tris-HCL pH8.8, and 20% SDS. We mixed the measured amounts into a conical vial. We then added 10% APS and TEMED and mixed carefully. ...

This question cannot be answered with this much of information. I am not sure why people are voting to keep it open

same with this:

3

Q: E. coli not growing in liquid medium

We are regularly doing bacterial transformation and subculture from plate to liquid media to extract DNA. This usually goes very well and is straightforward, but occasionally, the colonies that grew well on a plate, will not grow in liquid culture (with the same antibiotics that were in the plate...

molecular-biology microbiology plasmids

There are thousands of reasons for why a certain experiment may not work. Unless specific details are provided it is not possible to figure out the exact reason.

I don't understand why people ask questions and do not respond to questions about their methodology and so on.

I think the SDS-PAGE problem was not even recurrent. Could be a random error of mixing

I have no crystall ball which tells me what people did exactly in the lab

I think troubleshooting questions should be addressed only if the problem is recurrent and the OP has tried some troubleshooting themselves

And this entire issue of BioSE being too fast with closure and too hard on newbies: They should try asking a question such as "my computer crashed today" on so or superuser.

7:52 AM

@WYSIWYG Good. :-)

I agree. People should ask before they write questions. Will you answer this guy on meta?

@WYSIWYG Interesting paper on what?

8:14 AM

@FaheemMitha Anything at all. I am reading about redox regulated genes right now

8:34 AM

@WYSIWYG oh. You could look at my research paper if you wanted. How is your Bayesian Statistics?

8:52 AM

@FaheemMitha Can understand.. I find bayesian interesting

9:12 AM

@Chris I did post a comment. No patience to explain. In any case this topic has been discussed several times before.

@FaheemMitha Can you share a link to your paper?

@WYSIWYG Same here. :-)

9:27 AM

@Chris You have delete privileges right. Delete these two — 1 and 2

I have already voted to delete these two.

10:21 AM

@WYSIWYG I was planning on writing who on earth had not have this problem before, adding gallons of TEMED over the course of hours, ending up re-doing it after, just to find out the first one is hard as concrete while you are just pouring the second :-) refrained from doing so after I saw he had some sort of duplicate that did solidify

But yeah, borderline questions

No electrophysiologist will even consider asking why a cell wouldn't patch :-) Some things in science are more magic than science

@WYSIWYG - An interesting paper...

>:( bwuhahaha

Joking

What do you find interesting? Let me think, what was the most awesome thing I've read....

You're probably already all done being bored anyway ;-)

@AliceD I was reading a paper on redox controlled transcription of chloroplast genes.

And for a moment had gone to the bakery to get a cake for a friend on her bday.

@WYSIWYG and you wish a replacement?

:P

That sounds good haha

@AliceD I am yet to finish. And I am reading couple of more papers on the evolution of ATP/ADP exchangers

but in general

if you happened to read some interesting stuff then please share

10:33 AM

I think our disciplines are ummm different :)

I was, however, pointed to a few interesting papers I will be reading shortly.

Yeah that is fine. I am not looking for papers from my discipline. Anything that is interesting science.

Retinal implant clinical trial stuff

Have to dig them up first, though

No no.. no clinical trials. That is one thing that I am not very keen on reading

Hahaha

Nothing that is too anthropocentric

10:35 AM

But these are on making the blind see again

Playing God

:D

Haha.. well.. basic science interests me more. For others I can just have a summary from someone.

If you read that then tell me what happened at the end of it.

@AliceD See very anthropocentric

Had to google the term

I have started developing interest in protists and plants. Photosynthesis etc

anthropocentric= human centric

all the disease stuff

Well yes, much of my research has focused on (pre-)clinical stuff

absolutely

So yes, our interests are.. different :)

but I thought you work on neurophysiology

10:39 AM

I did on my previous postdoc

recording retinal responses from folks with a retinal implant

What about this quantum leap

?

Now I'm doing psychophysics

shift of interest?

@WYSIWYG I don't have it publicly online. I can send you an email. Either give me your email address, or send me an email at - faheem at faheem dot info.

i just got this opportunities

-s

10:41 AM

@FaheemMitha it should be on my SE profile

But psychophysics is the subjective approach, electrophysiology the objective

Two sides of the same I guess

@AliceD I am googling that term

@WYSIWYG ok

what?

psychophysics ?

It's generally regarded as psychology haha

yep.. is it like behavioural aspect of sensory physiology?

10:42 AM

One is biophysics, the other psychophysics

yes yes!

@WYSIWYG I don't see it.

google.com.au/…

@FaheemMitha I sent you a mail

@WYSIWYG ok

@AliceD IF you had the freedom of choosing any topic then what would you choose.

unbounded by other factors such as limitation of opportunities

10:49 AM

@WYSIWYG I got it, if you are Bharat. But you could have given a little context. If I get a mail I don't recognise, I tend to delete it immediately. Reflex reaction to spam.

I'll send you my paper right now.

Email sent.

@WYSIWYG did you get the email?

11:05 AM

@WYSIWYG a combination of both!

11:19 AM

@AliceD You're in Canberra? How is Australia doing these days from the pov of academic employment?

11:39 AM

@FaheemMitha I think Australia in general is not doing bad. I am loosely affiliated to the Australian National University, but I am not high up in the food chain honestly. Also, I am focusing my attention on Europe.

So I can't provide you with reliable info :)

@AliceD Europe for permanent positions?

@AliceD I wasn't really looking for any. :-)

@FaheemMitha Yes

is AU not good for permanent employment then? Or you just prefer Europe?

Oh I thought you were planning on coming over. If it is out of general interest; Ozzie is doing pretty well :)

Well we are moving back. I like Australia, I'd prefer the US though :)